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Hydration effects of moisturizing gel on normal skin: A pilot study

Joanna Xuan Hui Goh, Loh Teng-Hern Tan, Han Choi Yew, Priyia Pusparajah, Prithvy Lingham, Chiau Ming Long, Learn-Han Lee, Bey-Hing Goh Abstract - 482 PDF - 189


Moisturizers are topically applied agent which increasing moisture content in the skin to maintain skin integrity and youthful appearance. L-brand soothing gel (Tested gel) was developed for skin hydration. The objective of this study is to evaluate the benefits of daily usage of Tested gel amongst healthy young adults. This study also aimed to assess whether storage temperature may affect the efficacy of Tested gel. Changes in moisture content and texture index of the skin where the product was applied over a period of 8 days were assessed using non-invasive devices to measure the skin’s bioelectric impedance. Our results indicate that application of Tested gel produces a hydrating effect characterized by a significant increment in skin moisture and smoothness. There was no association found between storage temperature and efficacy of gel. The hydration effect of Tested gel diminishes gradually after application, but the hydration effect is cumulative upon daily usage for healthy young adults.

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Mahendra CK, Tan LTH, Yap WH, et al. An optimized cosmetic screening assay for UVB protective property of natural products. Prog Drug Discover Biomed Sci 2019; 2(1).

Proksch E, Brandner JM, and Jensen JM, The skin: an indispensable barrier. Exp Dermatol 2008. 17(12): 1063-1072.

Poet TS and McDougal JN, Skin absorption and human risk assessment. Chem Biol Interact 2002; 140(1): 19-34.

Lee SH, Jeong SK, and Ahn SK, An update of the defensive barrier function of skin. Yonsei Med J 2006; 47(3): 293-306.

Verdier‐Sévrain S and Bonté F, Skin hydration: A review on its molecular mechanisms. J Cosmet Dermatol 2007; 6(2): 75-82.

Rawlings AV and Harding CR, Moisturization and skin barrier function. Dermatol Ther 2004; 17: 43-48.

Harding C, Long S, Richardson J, et al. The cornified cell envelope: An important marker of stratum corneum maturation in healthy and dry skin. Int J Cosmet Sci 2003; 25(4): 157-167.

Scott IR and Harding CR. Filaggrin breakdown to water binding compounds during development of the rat stratum corneum is controlled by the water activity of the environment. Dev Biol 1986; 115(1): 84-92.

Saraf S. Formulating moisturizers using natural raw materials, in Treatment of Dry Skin Syndrome. 2012; Springer. p. 379-397.

Dal'Belo SE, Rigo Gaspar L, and Berardo Gonçalves Maia Campos PM. Moisturizing effect of cosmetic formulations containing Aloe vera extract in different concentrations assessed by skin bioengineering techniques. Skin Res Technol 2006; 12(4): 241-246.

Mahor G and Ali SA. Recent update on the medicinal properties and use of Aloe vera in the treatment of various ailments. Biosci Biotechnol Res Commun 2016; 9(2): 277-292.

Rosado C, Pinto P, and Rodrigues LM. Assessment of moisturizers and barrier function restoration using dynamic methods. Skin Res Technol 2009; 15(1): 77-83.

Loden M The clinical benefit of moisturizers. J Eur Acad Dermatol Venereol; 2005 19(6): 672-688.

Hadi H, Awadh A, Hanif N, et al. The investigation of the skin biophysical measurements focusing on daily activities, skin care habits, and gender differences. Skin Res Technol 2016; 22(2): 247-254.

Carville K, Leslie G, Osseiran‐Moisson R, et al. The effectiveness of a twice‐daily skin‐moisturising regimen for reducing the incidence of skin tears. Int Wound J 2014; 11(4): 446-453.

Kapoor S and Saraf S. Assessment of viscoelasticity and hydration effect of herbal moisturizers using bioengineering techniques. Pharmacogn Mag 2010; 6(24): 298.

Manvitha K and Bidya B. Aloe vera: A wonder plant its history, cultivation and medicinal uses. J Pharmacogn Phytochem 2014; 2(5): 85-88.

Kang M-C, Kim SY, Kim YT, et al. In vitro and in vivo antioxidant activities of polysaccharide purified from aloe vera (Aloe barbadensis) gel. Carbohyd Polym 2014; 99: 365-371.

Chun-hui L, Chang-hai W, Zhi-liang X, et al. Isolation, chemical characterization and antioxidant activities of two polysaccharides from the gel and the skin of Aloe barbadensis Miller irrigated with sea water. Process Biochem 2007; 42(6): 961-970.

Duansak D, Somboonwong J, and Patumraj S. Effects of Aloe vera on leukocyte adhesion and TNF‐α and IL‐6 levels in burn wounded rats. Clin Hemorheol Microcirc 2003; 29(3,4): 239-246.

Park M-Y, Kwon H-J, and Sung M-K. Evaluation of aloin and aloe-emodin as anti-inflammatory agents in aloe by using murine macrophages. Biosci Biotechnol Biochem 2009; 73(4): 828-832.

Vijayalakshmi D, Dhandapani R, Jayaveni S, et al. In vitro anti inflammatory activity of Aloe vera by down regulation of MMP-9 in peripheral blood mononuclear cells. J Ethnopharmacol 2012; 141(1): 542-546.

Nejatzadeh-Barandozi F. Antibacterial activities and antioxidant capacity of Aloe vera. Org Med Chem Lett 2013; 3(1): 5.

Im S-A, Oh S-T, Song S, et al. Identification of optimal molecular size of modified Aloe polysaccharides with maximum immunomodulatory activity. Int Immunopharmacol 2005; 5(2): 271-279.

Cellini L, Di Bartolomeo S, Di Campli E, et al. In vitro activity of A loe vera inner gel against H elicobacter pylori strains. Lett Appl Microbiol 2014; 59(1): 43-48.

Suksomboon N, Poolsup N, and Punthanitisarn S. Effect of Aloe vera on glycaemic control in prediabetes and type 2 diabetes: A systematic review and meta‐analysis. J Clin Pharm Ther 2016; 41(2): 180-188.

Suboj P, Babykutty S, Gopi DRV, et al. Aloe emodin inhibits colon cancer cell migration/angiogenesis by downregulating MMP-2/9, RhoB and VEGF via reduced DNA binding activity of NF-κB. Eur J Pharm Sci 2012; 45(5): 581-591.

Huang P-H, Huang C-Y, Chen M-C, et al. Emodin and aloe-emodin suppress breast cancer cell proliferation through ERα inhibition. Evid Based Complement Alternat Med 2013; 2013.

Yagi A, Egusa T, Arase M, et al. Isolation and characterization of the glycoprotein fraction with a proliferation-promoting activity on human and hamster cells in vitro from Aloe vera gel. Planta Med 1997; 63(01): 18-21.

Tarameshloo M, Norouzian M, Zarein-Dolab S, et al. Aloe vera gel and thyroid hormone cream may improve wound healing in Wistar rats. Anat Cell Biol 2012; 45(3): 170-177.

Femenia A, Garcı́a-Pascual P, Simal S, et al. Effects of heat treatment and dehydration on bioactive polysaccharide acemannan and cell wall polymers from Aloe barbadensis Miller. Carbohyd Polym 2003; 51(4): 397-405.

Miranda M, Maureira H, Rodriguez K, et al, Influence of temperature on the drying kinetics, physicochemical properties, and antioxidant capacity of Aloe Vera (Aloe Barbadensis Miller) gel. J Food Eng 2009; 91(2): 297-304.

Fox LT, Du Plessis J, Gerber M, et al. In Vivo skin hydration and anti-erythema effects of Aloe vera, Aloe ferox and Aloe marlothii gel materials after single and multiple applications. Pharmacogn Mag 2014; 10(Suppl 2): S392.

Constantin M-M, Poenaru E, Poenaru C, et al. Skin hydration assessment through modern non-invasive bioengineering technologies. Maedica 2014; 9(1): 33.

Shelton RM. Aloe vera: Its chemical and therapeutic properties. Int J Dermatol 1991; 30(10): 679-683.

Jemec GB and Na R. Hydration and plasticity following long-term use of a moisturizer: a single-blind study. Acta Derm Venereol 2002; 82(5).

Chandegara V and Varshney A. Aloe vera L. processing and products: A review. Int J Med Aromat Plants 2013; 3: 492-506.

Choi S and Chung M-H. A review on the relationship between Aloe vera components and their biologic effects. Semin Integr Med 2003; 1(1): 53-62.

Yanovski SZ, Hubbard VS, Heymsfield SB, et al. Bioelectrical impedance analysis in body composition measurement: National Institutes of Health technology assessment conference statement. Am J Clin Nutr 1996; 64(3): 524S-532S.

Li F, Conroy E, Visscher M, et al. The ability of electrical measurements to predict skin moisturization. II. Correlation between one-hour measurements and long-term results. J Cosmet Sci 2001; 52(1): 23-33.

Berardesca E, Cosmetics EGfEMo, and Products OT. EEMCO guidance for the assessment of stratum corneum hydration: electrical methods. Skin Res Technol 1997; 3(2): 126-132.

De Paepe K, Derde MP, Roseeuw D, et al. Claim substantiation and efficiency of hydrating body lotions and protective creams. Contact Dermatitis 2000; 42(4): 227-234.

Ehlers C, Ivens U, Møller M, et al. Females have lower skin surface pH than men: A study on the influence of gender, forearm site variation, right/left difference and time of the day on the skin surface pH. Skin Res Technol 2001; 7(2): 90-94.

Buffa R, Mereu E, Comandini O, et al. Bioelectrical impedance vector analysis (BIVA) for the assessment of two-compartment body composition. Eur J Clin Nutr 2014; 68(11): 1234.


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